The MEDSynth Lab
Organic synthesis
MEDSynth employs state-of-the-art techniques such as parallel chemistry, solid phase and microwave-assisted organic synthesis (MAOs), and classical solution-phase organic synthesis to increase the outputs. The MEDSynth labs are fully equipped for both liquid and solid-phase syntheses.
Specifically :
- Teledyne Isco CombiFlash System
- Medium Pressure Liquid Chromatography (MPLC Buchi 688)
- High-pressure PARR® reactor (high-pressure reactions)
- Kugelrohr distillation apparatus
- Analytical HPLC (Merck-Hitachi L-6200, double pump system, UV detector, autosampler)
- Analytical Perkin Elmer UHPLC FLEXAR FX-15 Platform
- UltiMateTM 3000 Semi-Preparative System
- 500 mL Reactor-Ready Controlled Lab reactor (temperature range: -70°C/+230°C; pressure range: 10 mbar-0.05 bar; max rate: 500 rpm; software: AVA) Radleys UK
Our group have access to other facilities at the Department of Drug Science and Technology (University of Torino), due to internal collaborations:
- 600 MHz Jeol NMR
- Milestone MicroSYNTH microwave oven (two units)
- Milestone SinthWave microwave oven
- Waters Binary pump 1525EF, 2998 Diode array detector, 2424 ELS detector, WFC III fraction collector.
- G.E. Akta Purifier 10/100 System
- Waters FractionLink Autopurification System (Binary pump 2525, 2767 Autosampler/fraction collector, 2996 Diode array detector, ZQ ESCi- MS detector)
- Agilent 6850 GC System equipped with 5973 Mass Selective Detector
500 mL Reactor-Ready Controlled Lab reactor
Analytical Perkin Elmer UHPLC FLEXAR FX-15 Platform
UltiMateTM 3000 Semi-Preparative System
ADME & Drug-like properties
Once the compounds have been synthesized, the ADME facility is responsible for the following profilation:
- Chemical-physical properties of the new compounds, in particular thermodynamic solubility and LogD measurement
- Evaluation of the stability of the compounds in different media (PBS, SGF, SIF, serum)
- Investigations of microsomal metabolism and stability in S9 fractions
- Serum protein binding
- Evaluation of in vitro enzyme-inhibiting activity
To perform our analysis, the ADME laboratory is equipped with:
- Analytical Perkin Elmer UHPLC FLEXAR FX-15 Platform
- UV-visible spectrophotometer VARIAN 50 BIO
- Thermomixer Shaker BIOER MB-102
- Centrifuge ALC PK 110
- Refrigerated Microlitre Centrifuge HERMLE Z216MK
- Incubator NUVE EN032
Analytical Perkin Elmer UHPLC FLEXAR FX-15 Platform
MEDSynth Group in the Metaverse environment of Nanome
In silico studies
MEDSynth group is also able to perform in silico drug design through the use of different devices.
- Six Oculus META QUEST 2 supporting Nanome software for the immersive studies of small molecule-protein interactions.
- Centos 7 molecular Docking station: GOLD, Maestro, HADDOCK.